TLC AND HPLC Fingerprint development of Aegle marmelos Corr. And its polyherbal marketed formulations

Chromatographic fingerprint analysis of herbal drugs represents a comprehensive qualitative approach for the purpose of species authentication, evaluation of quality and ensuring the consistency and stability of herbal drugs and their related products. In the present research article TLC and HPLC Chromatogram of Aegle marmelos Corr is taken as standard for comparing its fingerprinting profile with three marketed formulations containing Aegle marmelos Corr as one ingredient. Bilva patra showed four spots at 0.89, 0.75, 0.65, and 0.52. in thin layer chromatography. Bilwadi churna also showed three spots at 0.89 ,0.80 and 0.63. Pushyanug churna showed two spots at 0.89 and 0. .Whereas Gangadhar churna showed two spots of which only one spot is matching with Bilva Patra (0.63). On the inspection of various chromatogram of Bilva Patra and its formulations in Polyherbal drugs, gives various peaks from 4.8-10.. It seems that the peaks at 4.8,5.8,and 9.2 may be beneficial for standardizing the Bilva Patra in the polyherbal formulations,but when the chromatogram of polyherbal formulations have been been recorded , it was found that the peaks obtained at 9.2 have been retarded to a negligible amount, but only the peak of 5.8 min can be taken as standard.